Rapid Identification of Emetic Bacillus cereus by Immunochromatography
نویسندگان
چکیده
منابع مشابه
Identification of emetic toxin producing Bacillus cereus strains by a novel molecular assay.
Bacillus cereus causes two types of gastrointestinal diseases: emesis and diarrhea. The emetic type of the disease is attributed to the heat-stable depsipeptide cereulide and symptoms resemble Staphylococcus aureus intoxication, but there is no rapid method available to detect B. cereus strains causing this type of disease. In this study, a polymerase chain reaction (PCR) fragment of unknown fu...
متن کاملEmetic toxin-producing strains of Bacillus cereus show distinct characteristics within the Bacillus cereus group.
One hundred representative strains of Bacillus cereus were selected from a total collection of 372 B. cereus strains using two typing methods (RAPD and FT-IR) to investigate if emetic toxin-producing hazardous B. cereus strains possess characteristic growth and heat resistance profiles. The strains were classified into three groups: emetic toxin (cereulide)-producing strains (n=17), strains con...
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Despite of improving levels of hygiene, the incidence of registered food borne diseases has been at the same level for many years: there were 40 to 90 epidemics in which 1000-9000 persons contracted food poisoning through food or drinking water in Finland. Until the year 2004 salmonella and campylobacter were the most common bacterial causes of food borne diseases, but in years 2005-2006 Bacill...
متن کاملFood–bacteria interplay: pathometabolism of emetic Bacillus cereus
Bacillus cereus is a Gram-positive endospore forming bacterium known for its wide spectrum of phenotypic traits, enabling it to occupy diverse ecological niches. Although the population structure of B. cereus is highly dynamic and rather panmictic, production of the emetic B. cereus toxin cereulide is restricted to strains with specific genotypic traits, associated with distinct environmental h...
متن کاملSemiautomated metabolic staining assay for Bacillus cereus emetic toxin.
This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, only B. cereus was cytotoxic.
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ژورنال
عنوان ژورنال: Biocontrol Science
سال: 2011
ISSN: 1884-0205,1342-4815
DOI: 10.4265/bio.16.41